Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody

TTF-1 / NKX2-1 antibody

Boster Bio Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody (Catalog # M01322). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01322
Size: 100ug/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC

Product Name

Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody

View all TTF-1 / NKX2-1 Antibodies

SKU/Catalog Number

M01322

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody (Catalog # M01322). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01322)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM150

Isotype

IgG1, kappa

Immunogen

Rat full length TTF-1 recombinant protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01322 is reactive to NKX2-1 in Human, Mouse, Rat

Applications

M01322 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

38.596kDa

Background of TTF-1 / NKX2-1

Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For NKX2-1 (Source: Uniprot.org, NCBI)

Gene Name

NKX2-1

Full Name

Homeobox protein Nkx-2.1

Weight

38.596kDa

Superfamily

NK-2 homeobox family

Alternative Names

BCH; benign chorea; BHC; Homeobox protein NK-2 homolog A; homeobox protein Nkx-2.1; NK2 homeobox 1; NK-2 homolog A; NKX2.1; NKX2-1; NKX2A; NKX2ANKX2.1; T/EBP; Thyroid nuclear factor 1; Thyroid transcription factor 1BHC; TITF1; TITF1TEBP; TTF1; TTF-1; TTF1BCH; TTF-1NK-2 NKX2-1 BCH, BHC, NK-2, NKX2.1, NKX2A, NMTC1, T/EBP, TEBP, TITF1, TTF-1, TTF1 NK2 homeobox 1 homeobox protein Nkx-2.1|NK-2 homolog A|homeobox protein NK-2 homolog A|thyroid nuclear factor 1|thyroid transcription factor 1|thyroid-specific enhancer-binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NKX2-1, check out the NKX2-1 Infographic

NKX2-1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NKX2-1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal Antibody

Question

I see that the anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal antibody M01322 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-12

Question

Is this M01322 anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal antibody reactive to the isotypes of NKX2-1?

Verified Customer

Verified customer

Asked: 2018-11-05

Answer

The immunogen of M01322 anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal antibody is Rat full length TTF-1 recombinant protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-05

Question

I am interested in to test anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal antibody M01322 on rat left lobe of thyroid gland for research purposes, then I may be interested in using anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal antibody M01322 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-08

Answer

The products we sell, including anti-TTF-1 / NKX2.1 (Thyroid & Lung Epithelial Marker) Monoclonal antibody M01322, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-08

Order DetailsPrice
M01322-100ug

100 μg

$468

Free Secondary Antibody

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M01322
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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